Performing ELISAs is an efficient method to test many samples in different dilutions in one experiment. Monitoring of cumulative antibody production throughout the culture period Advantages of antibody production and isotype analysis by ELISA: This method can be used to screen for the immunogenicity and immunomodulatory properties of compounds and biologicals with regards to B cell responses. With an antibody ELISA, the concentration of antibodies in serum, plasma or culture medium is measured. We can assist in providing an insight into B cell antibody production and the isotypes thereof. Furthermore, the formation of anti-drug-antibodies can severely impact drug effectiveness. Do Not use in any diagnostic or therapeutic applications.Antibody production by B cells is one of the main targets of many vaccination strategies. If you are interested in our whole cell immunization service, please do not hesitate to inquire us for more details.Īll listed services and products are For Research Use Only. We are flexible enough to design and perform the unique protocols to meet your specific needs. One of the best method for anti-membrane protein antibody discoveryĪs one of the well-recognized experts who are professional in applying advanced phage display technologies for a broad range of antibody discovery, Creative Biolabs is glad to provide the service of antibody production via whole cell immunization method. Naïve conformation and modification of interest target In addition, we can also use this strategy to discovery single domain antibodies with high affinity and stability.įig.1 The flowchart of monoclonal antibodies production.įeatured Advantages of Whole Cell Immunization Creative Biolabs can employ either hybridoma, phage display or other novel techniques for the selection and validation of specific monoclonal antibody. After screening using the target cells and counter-screening using negative cells (the same cell line but do not express the target protein), the target-specific binders can be selected for further validation. By generating the special cell lines that overexpress the target proteins, their natural conformation and also the modifications can be maintained, our scientist can therefore lay a good foundation for the high-quality antibody discovery at the early stage of animal immunization. These cell-surface proteins may lose their native conformation during the regular purification process and result in the challenges for discovering specific mAb against the conformational epitope. Compared with other immunization strategies, whole cell immunization has been successfully used for the mAb generation against cell-surface proteins as membrane and membrane-associated protein. The whole cell immunization has been widely used for the antibodies generation. Besides, this strategy is also a suitable method to identify new biomarkers from the surface proteins of tumor cell lines, thus can contribute to the cancer diagnosis and also the development of novel therapeutic reagents. Compared with the normal immunization strategies, this strategy can benefit from higher immunogenicity, naïve antigen conformation and avoid the complex purification process, which is specifically suitable for novel antibody discovery against membrane protein (extracellular domain). Whole cell immunization is a novel strategy that the antigen overexpression cell line can be used as the immunogen. To meet our clients’ various requirement in antibody discovery projects our scientists are able to provide the whole cell immunization strategy for our customers all over the world. HighlightsĪntibody Production by Whole Cell ImmunizationĬreative Biolabs has been a long-term expert in the field of antibodies discovery. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. Cell-based Membrane Protein Expression and PurificationĬreative Biolabs is offering the most comprehensive services for antibody development projects.Cell-free Membrane Protein Expression and Purification.Membrane Protein Solubilization and Stabilization.Manufacturability Assessment and Optimization.SIAT ® Immunogenicity Assessment Services.CreMap™ Epitope Mapping & Discovery Services.Dual-Polarization Interferometry (DPI) Services.Differential Scanning Calorimetry (DSC) Services.Isothermal Titration Calorimetry (ITC) Services.De Novo Human/Humanized Antibody Sequencing Service.Magic™ Human Antibody Production Using Transgenic Mice.Bovine Ultralong CDR3 Antibody Humanization.Antibody Off-target Identification and Specificity Profiling Service. Biomolecular Interaction Analysis Services.Epitope Prediction for the Identification of Antibody Epitopes.Epitope Mapping by X-ray Crystallography.Phage Display & Antibody Library Services.
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